Livestock has recently been identified as a new reservoir of methicillin-resistant Staphylococcus aureus MRSA. Most isolates belong to ST398 and are non-typeable with PFGE using SmaI, making it difficult to study transmission and outbreaks.Therefore, a new PFGE using Cfr9I, an neoschizomer of SmaI was optimized and evaluated to investigate ST398 isolates.Results: After optimizing and evaluating the Cfr9I PFGE, clear and reproducible banding patterns were obtained from all previously non-typeable MRSA NTSmaI -MRSA isolates. The PFGE patterns of ST398 isolates showed more diversity than with spa-typing and/or MLST.The PFGE results showed diversity within and between the two most prevalent spa-types of NTSmaI -MRSA t011 and t108. No match was found, when comparing banding patterns of the NTSmaI -MRSA with 700 different PFGE types, obtained with SmaI digestion, in our database of more than 4000 strains.Furthermore, possible transmission among veterinarians and their family members was investigated and an outbreak of ST398 MRSA in a residential care facility was confirmed with the Cfr9I PFGE.
MRSA, Farm Animals and Human Infection
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